Method: LC-MS/MS (Liquid Chromatography/Tandem Mass Spectrometry) with creatinine normalization by Jaffe Reaction (CLIA 17D0919496); not cleared or approved by the FDA. Results reported in µg/g creatinine. Not a stand-alone diagnosis; should be interpreted in clinical context.
A derived biomarker is a value that is calculated from other directly measured biomarkers rather than being measured directly in the lab.
Key benefits of 2-Hydroxyethyl Mercapturic Acid (HEMA) testing
- Ethylene oxide and vinyl chloride exposure tracking
- combined sterilization chemical and plastics burden assessment
What is 2-Hydroxyethyl Mercapturic Acid (HEMA)?
HEMA is a urinary mercapturic acid metabolite of both ethylene oxide (used in medical device sterilization) and vinyl chloride (used in PVC production and found in cigarette smoke). Measured via LC-MS/MS.
Why is 2-Hydroxyethyl Mercapturic Acid (HEMA) important?
Both ethylene oxide and vinyl chloride are classified as known human carcinogens (per IARC Group 1). While high-level exposures are primarily occupational, lower-level exposures occur through tobacco smoke, sterilized medical products, and PVC-containing environments. HEMA serves as a combined biological marker for both.
What insights will I get?
Your HEMA level may reflect tobacco smoke, exposure to recently sterilized medical products, or contact with PVC-containing materials. Detectable levels in non-smokers without known occupational exposure may suggest environmental sources worth investigating.





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