Method: LC-MS/MS (Liquid Chromatography/Tandem Mass Spectrometry) with creatinine normalization by Jaffe Reaction (CLIA 17D0919496); not cleared or approved by the FDA. Results reported in µg/g creatinine. Not a stand-alone diagnosis; should be interpreted in clinical context.
A derived biomarker is a value that is calculated from other directly measured biomarkers rather than being measured directly in the lab.
Key benefits of N-Acetyl (Carbomethyl) Cysteine (NAE) testing
- Acrylamide dietary exposure tracking
- high-heat cooked food and tobacco smoke chemical burden assessment
What is N-Acetyl (Carbomethyl) Cysteine (NAE)?
NAE is a urinary metabolite of acrylamide, a compound formed during high-heat cooking of starchy foods via the Maillard reaction. Tobacco smoke is an additional major source. Measured via LC-MS/MS.
Why is N-Acetyl (Carbomethyl) Cysteine (NAE) important?
Acrylamide is classified as a probable human carcinogen. It forms naturally in fried, baked, and roasted starchy foods - french fries, potato chips, toast, crackers, and some cereals are common dietary sources. Urinary NAE provides a direct personal measure of dietary and smoke-related acrylamide burden.
What insights will I get?
Your NAE level may indicate dietary patterns heavy in fried or high-heat starchy foods, or tobacco smoke exposure. Elevated levels may prompt dietary adjustments - reducing deep-fried foods, heavily toasted bread, and ultra-processed starchy snacks - which may be reflected in follow-up testing.





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